Laboratory of Reproductive Medicine

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About us

Laboratory of Reproductive medicine is focused on spermatogenesis, oocyte maturation, mechanisms of fertilization and pre-implanted embryonic development in vitro. Mice (Mus musculus) are utilized as a biological model allowing transfer of our results onto further research of human reproduction. In addition to in vitro experiments, mouse model offers in vivo testing of selected environmental polutants. Moreover, analysis of human gametes and body fluids of reproductive system takes place in Laboratory of Reproductive Medicine.

What are we focused on?

  • Epigenetic regulation of fertilization and early embryonic development.
  • Influences negatively affecting sperm quality and fertilization ability.
  • Role of ubiquitin-proteasomal system in epigenetics.
  • Involvement of hydrogen sulfide, one of gasotransmitters, in epigenetic changes.
  • Evaluation of impact of endocrine disruptors on mammalian reproduction.

What we can do?

  • In vitro oocyte maturation (IVM), in vitro fertilization (IVF) and embryo culture (IVC).
  • Flow cytometry and sorting of sperm (FACSAria, FACSVerse, BD Bioscience).
  • Intracytoplasmic sperm injection (ICSI).
  • Immunocytochemistry of oocytes and embryos.
  • Epi-fluorescent and confocal laser-scanning microscopy, live cell imaging (Olympus, IX83, Tokai Hit).
  • Western Blotting (Biorad).

Our partners

  • Institute of Animal Sciences, Prague, Czech Republic (prof. J. Petr)
  • Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Czech Republic (T. Žalmanová, Ph.D., K. Hošková, Ph.D.)
  • Health & Biotechnology (SaBio) group, Spanish Wildlife Research Institute (IREC; CSIC-UCLM-JCCCM), Spain (prof. J. Garde), http://www.sabio-irec.com/
  • Division of Animal Sciences, University of Missouri, Columbia, MO, USA (prof. P. Šutovský, Dr.h.c.)
  • Régulation des Signaux de Division Team, Université Lille1, Sciences et Technologies (prof. J.-F. Bodart), Villeneuve dAscq, France
  • Department of Animal Physiology, Slovak University of Agriculture, Nitra, Slovak Republic (assoc. prof. A. Kolesárová)
  • Division of Biotechnology, Chonbuk National University, Jeonbuk, Republic of Korea (Y. J. Yi, PhD.)

Releated Research themes

Contact

Biomedical Center
alej Svobody 1655/76
323 00 Plzen
T: +420 377 593 808
E: This email address is being protected from spambots. You need JavaScript enabled to view it.

Members

Publications and Patents

  • del Barco-Trillo J, García-Álvarez O, Soler AJ, Tourmente M et al.: A cost for high levels of sperm competition in rodents: increased sperm DNA fragmentation. Proceedings B. 2016. DOI:10.1089/rspb.2015.2708
  • García-Álvarez O, Maroto-Morales A, Jiménez-Rabadán P, Ramón M et al.: Effect of different media additives on capacitation of frozen-thawed ram spermatozoa as a potential replacement for estrous sheep serum. Theriogenology 2015, 84:948-955. DOI:10.1016/j.theriogenology.2015.05.032
  • García-Álvarez O, Maroto-Morales A, Ramón M, del Olmo E et al.: Dynamic of sperm subpopulations based on motility and membrane status in thawed ram spermatozoa incubated under conditions that support in vitro capacitation and fertilization. Reproduction, Fertility and Development 2014, 26:725-732. DOI:10.1071/RD13034
  • Nevoral J, Petr j, Gelaude A, Bodart JF et al.: Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes. PLOS ONE 07/2014; 9(7):e99613. DOI:10.1371/journal.pone.0099613
  • Nevoral J, Sutovsky P: Involvement of epigenetic changes and ubiquitin-dependent proteolysis in the pronuclear development of mammalian zygote. In: Heide Schatten (ed.): Animal Models and Human Reproduction. New York: John Wiley & Sons, 2016. In Press.
  • Nevoral J, Zalmanova T, Bodart JF, Petr J et al.: Endogenously produced hydrogen sulfide is involved in porcine oocyte maturation in vitro. Nitric Oxide: Biology and Chemistry 12/2015, 51(12). DOI:10.1016/j.niox.2015.09.007.

Contact

Charles Univerzity
Faculty of Medicine in Pilsen
Biomedical Center

alej Svobody 1655/76
323 00 Plzeň – Severní Předměstí

T: +420 377 593 810
E: This email address is being protected from spambots. You need JavaScript enabled to view it.

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