Laboratory of Antibiotic Resistance and applications of Mass Spectrometry in Microbiology

About us

Scientific activities of Laboratory of Antibiotic Resistance and Application of Mass spectrometry in Microbiology (ARAMM) are mainly focused on mechanisms of resistance to antibiotics, especially to expanded-spectrum cephalosporins and carbapenems, in Gram-negative bacteria (Enterobacteriaceae, Pseudomonas spp.). The research projects aim to execute molecular epidemiology of factors affecting dissemination of multi-drug resistance among nosocomial pathogens. Methodological approaches, also, include analysis of genetic carriers of resistance determinants and whole-genome sequencing. The second main objective of the research projects is intending in the development of new techniques for the detection and study of resistance mechanisms using MALDI-TOF mass spectrometry and Raman spectroscopy. In 2011, the members of our group demonstrated that MALDI-TOF mass spectrometry can directly detect carbapenemase activity. Also, recently, we patented a method for the identification of β-lactamases in clinical isolates by MALDI-TOF mass spectrometry, based on their molecular weight.

Releated Research themes


Biomedical Center
alej Svobody 1655/76
323 00 Plzeň – Severní Předměstí


  • Prof. Jaroslav Hrabák, Ph.D., M.Sc. – Research Group Leader
  • Jana Amlerová, M.D.
  • Tamara Bergerová, M.D.
  • Ibrahim Bitar, Ph.D.
  • Monika Dolejská, Ph.D., M.Sc.
  • Miroslava Chalupová, M.D.
  • Kateřina Chudějová, M.Sc.
  • Vladislav Jakubů, M.Sc.
  • Helena Janouškovcová, M.D.
  • Dana Králová, DiS.
  • Lucie Kraftová, B.Sc.
  • Constantinos Papagiannitsis, Ph.D.
  • Veronika Pašková, M.Sc.
  • Anna Skálová, M.D.
  • Radka Walková, M.D.
  • Assoc. Prof. Helena Žemličková, M.D., Ph.D.

We offer

  • DNA sequencing, including capillary and whole-genome sequencing, and metagenomic analysis
  • Molecular typing of Gram-negative bacteria
  • Detection of resistance mechanisms in Gram-negative bacteria (β-lactamases, mechanisms of resistance in fluroquinolones, aminoglycosides, colistin, etc.)
  • Applications of mass spectrometry in microbiology
  • Applications of Raman spectroscopy in microbiology

Publications and Patents



Charles University
Faculty of Medicine in Pilsen
Biomedical Center

alej Svobody 1655/76
323 00 Plzeň – Severní Předměstí

T: +420 377 593 810
E: This email address is being protected from spambots. You need JavaScript enabled to view it.


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