Two members of Laboratory of Cancer Treatment and Tissue Regeneration are coauthors of two scientific articles published in in Nature Protocols

Pavel Vodicka, M.D., PhD and Alena Opattova, PhD, from Laboratory of Cancer Treatment and Tissue Regeneration Bimedical Center in Pilsen and Institute of Experimental Medicine of the Czech Academy of Sciences, Prague are coauthors of two scientific articles published in in Nature Protocols (IF 11.360).

Both papers „Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results“ and „An optimized comet-based in vitro DNA repair assay to assess base and nucleotide excision repair activity“ were created as a result of longlasting intensive collaboration of international team od scientists. The working effort of many scientific groups on field of Comet assay was crowned by obtaining of COST: hCOMET - The comet assay as a human biomonitoring tool (http://www.hcomet.eu). On the base of this project was devising the standard protocol, for both DNA damage and DNA repair measurement, enabling reproducibility widely usability of comet assay in human biomonitoring studies. Pooled analyzes of future studies should be therefore facilitated. Moreover, questions connected with application of the assay in different human cell types using different storage conditions and isolation techniques were also addressed.

The paper "Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results" represent guidelines to improve the description, analysis, and reporting of experimental data generated using the comet assay (https://www.nature.com/articles / s41596-020-0398-1).

The paper "An optimized comet-based in vitro DNA repair assay to assess base and nucleotide excision repair activity" represents a detailed protocol of the method for functional measurement of base and nucleotide excision repair activity, with many applications in human biomonitoring, in in vitro and in vivo studies, as well as in intervention studies (https://www.nature.com/articles/s41596-020-0401-x).

MUDr. Ludmila Vodičková, CSc.

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