DNA damage response and DNA repair during mouse oocyte maturation

The Laboratory of Reproductive Medicine presents a lecture by Dr. Petr Šolc – Institute of Animal Physiology and Genetics, Czech Academy of Sciences on Wednesday, May 9th, 2018, 10 a.m. in seminar room of Biomedical Center.

The Petr Solc’s lecture has been canceled. The lecture will be postponed and alternative term will be announced.

Because low levels of DNA double strand breaks (DSBs) appear not to activate the ATM-mediated prophase I checkpoint in full-grown oocytes, there may exist mechanisms to protect chromosome integrity during meiotic maturation. Using live imaging we demonstrate that low levels of DSBs induced by the radiomimetic drug Neocarzinostatin (NCS) increase the incidence of chromosome fragments and lagging chromosomes but do not lead to APC/C activation and anaphase onset delay. The number of DSBs, represented by γH2AX foci, significantly decreases between prophase I and metaphase II in both control and NCS-treated oocytes. Transient treatment with NCS increases >2-fold the number of DSBs in prophase I oocytes, but less than 30% of these oocytes enter anaphase with segregation errors. MRE11, but not ATM, is essential to detect DSBs in prophase I and is involved in H2AX phosphorylation during metaphase I. Inhibiting MRE11 by mirin during meiotic maturation results in anaphase bridges and also increases the number of γH2AX foci in metaphase II. Compromised DNA integrity in mirintreated oocytes indicates a role for MRE11 in chromosome integrity during meiotic maturation.

The lecture will be about 45 min., accompanied by small refreshment


Charles University
Faculty of Medicine in Pilsen
Biomedical Center

alej Svobody 1655/76
323 00 Plzeň – Severní Předměstí

T: +420 377 593 810
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