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About us

Laboratory of Reproductive medicine is focused on spermatogenesis, oocyte maturation, mechanisms of fertilization and pre-implanted embryonic development in vitro. Mice (Mus musculus) are utilized as a biological model allowing transfer of our results onto further research of human reproduction. In addition to in vitro experiments, mouse model offers in vivo testing of selected environmental polutants. Moreover, analysis of human gametes and body fluids of reproductive system takes place in Laboratory of Reproductive Medicine.

What are we focused on?

• Epigenetic regulation of fertilization and early embryonic development.
• Influences negatively affecting sperm quality and fertilization ability.
• Role of ubiquitin-proteasomal system in epigenetics.
• Involvement of hydrogen sulfide, one of gasotransmitters, in epigenetic changes.
• Evaluation of impact of endocrine disruptors on mammalian reproduction.

What we can do?

• In vitro oocyte maturation (IVM), in vitro fertilization (IVF) and embryo culture (IVC).
• Flow cytometry and sorting of sperm (FACSAria, FACSVerse, BD Bioscience).
• Intracytoplasmic sperm injection (ICSI).
• Immunocytochemistry of oocytes and embryos.
• Epi-fluorescent and confocal laser-scanning microscopy, live cell imaging (Olympus, IX83, Tokai Hit).
• Western Blotting (Biorad).

Our partners

• University of Castilla-La Mancha, Spain 
• Constantine the Philosopher University in Nitra, Slovakia 
• Institute of Molecular and Cellular Biology of Rosario, Argentina 
• University of Missouri, USA 
• Chungbuk National University, South Korea 
• Institute of Animal Science, Czech Republic 
• Institute of Animals Physiology and Genetics, Czech Republic 
• 1st Faculty of Medicine, Charles University, Czech Republic 

Releated Research themes

• Regenerative Medicine
• Proteomics

Contact

Biomedical Center
alej Svobody 1655/76
323 00 Pilsen – Severní Předměstí
Telefon: +420 377 593 808
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. 
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Members

• Assoc. Prof. Jan Nevoral, M.Sc., Ph.D. (ResearchGate) – Research Group Leader
• Hedvika Řimnáčová, M.Sc., Ph.D.
• Václav Rúčka, M.Sc.
• Miki Shavit, M.Sc.
• Gabriela Přibáňová, M.Sc.
• prof. Milena Králíčková, M.D., Ph.D. – Senior Leader
• and pre-graduate students

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Publications and Patents

• Řimnáčová, H., Moravec, J., Štiavnická, M., Havránková, J., Monsef, L., Hošek, P., Prokešová, Š., Žalmanová, T., Fenclová, T., Petr, J., Králíčková, M., Nevoral, J. 2022. Evidence of endogenously produced hydrogen sulfide (H2S) and persulfidation in male reproduction. Scientific Reports. 12(1), 11426. doi: 10.1038/s41598-022-15360-x.
• Iniesta-Cuerda, M., Havránková, J., Řimnáčová, H., García-Álvarez, O., Nevoral, J. 2022. Male SIRT1 insufficiency leads to sperm with decreased ability to hyperactivate and fertilize. Reproduction in Domestic Animals. 5, 72–77. doi: 10.1111/rda.14172.
• Nevoral, J., Havránková, J., Kolinko, Y., Prokešová, Š., Fenclová, T., Monsef, L., Žalmanová, T., Petr, J., Králíčková, M. 2021. Exposure to alternative bisphenols BPS and BPF through breast milk: Noxious heritage effect during nursing associated with idiopathic infertility. Toxicology and Applied Pharmacology. 413, 115409. doi: 10.1016/j.taap.2021.115409.
• Řimnáčová, H., Štiavnická, M., Moravec, J., Chemek, M., Kolinko, Y., García-Álvarez, O., Mouton, P. R., Trejo, A. M. C., Fenclová, T., Eretová, N., Hošek, P., Klein, P., Králíčková, M., Petr, J., Nevoral, J. 2020. Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice. Reproductive Biology and Endocrinology. 18(1), 56. doi: 10.1186/s12958-020-00596-x.
• Nevoral, J., Landsmann, L., Štiavnická, M., Hošek, P., Moravec, J., Prokešová, Š., Řimnáčová, H., Koutná, E., Klein, P., Hošková, K., Žalmanová, T., Fenclová, T., Petr, J., Králíčková, M. 2019. Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression. Journal of Animal Science and Biotechnology. 10, 67. doi: 10.1186/s40104-019-0372-3.
• Nevoral, J., Žalmanová, T., Hošková, K., Štiavnická, M., Hošek, P., Petelák, A., Petr, J. 2018. Involvement of K+ATP and Ca2+ channels in hydrogen sulfide-suppressed ageing of porcine oocytes. Biological Research. 51(1), 38. doi: 10.1186/s40659-018-0187-2.
• Nevoral, J., Rúčka, V., Klein, P., Žalmanová, T., Hošková, K., Prokešová, Š., Štiavnická, M., Petr, J., Králíčková, M. 2018. The interplay of mouse and porcine biomodels for endocrine disruptor reproductive impairment. Slovak Journal of Animal Science. 51(4), 161–170. ISSN 1337-9984.
• Nevoral, J., Kolinko, Y., Moravec, J., Žalmanová, T., Hošková, K., Prokešová, Š., Klein, P., Ghaibour, K., Hošek, P., Štiavnická, M., Řimnáčová, H., Tonar, Z., Petr, J., Králíčková, M. 2018. Long-term exposure to very low doses of bisphenol S affects female reproduction. Reproduction. 156(1), 47–57. doi: 10.1530/REP-18-0092.
• Adámková, K., Yi, Y.-J., Petr, J., Žalmanová, T., Hošková, K., Jelínková, P., Moravec, J., Králíčková, M., Sutovský, M., Sutovský, P., Nevoral, J. 2017. SIRT1-dependent modulation of methylation and acetylation of histone H3 on lysine 9 (H3K9) in the zygotic pronuclei improves porcine embryo development. Journal of Animal Science and Biotechnology. 8, 83. doi: 10.1186/s40104-017-0214-0.
• Štiavnická, M., Abril-Parreño, L., Nevoral, J., Králíčková, M., García-Álvarez, O. 2017. Non-Invasive Approaches to Epigenetic-Based Sperm Selection. Medical science monitor: International medical journal of experimental and clinical research. 23, 4677–4683. doi: 10.12659/msm.904098.
• Žalmanová, T., Hošková, K., Nevoral, J., Adámková, K., Kott, T., Šulc, M., Kotíková, Z., Prokešová, Š., Jílek, F., Králíčková, M., Petr, J. 2017. Bisphenol S negatively affects the meotic maturation of pig oocytes. Scientific Reports. 7(1), 485. doi: 10.1038/s41598-017-00570-5.
• Nevoral, J., Sutovský, P. 2016. Involvement of epigenetic changes and ubiquitin-dependent proteolysis in the pronuclear development of mammalian zygote. In: Heide Schatten (ed.): Animal Models and Human Reproduction. New York: John Wiley & Sons.
• Nevoral, J., Bodart, J.-F., Petr, J. 2016. Gasotransmitters in Gametogenesis and Early Development: Holy Trinity for Assisted Reproductive Technology-A Review. Oxidative Medicine and Cellular Longevity. 1730750. doi: 10.1155/2016/1730750.
• Nevoral, J., Žalmanová, T., Zámostná, K., Kott, T., Kučerová-Chrpová, V., Bodart, J.-F., Gélaude, A., Procházka, R., Orsák, M., Šulc, M., Klein, P., Dvořáková, M., Weingartová, I., Víghová, A., Hošková, K., Krejčová, T., Jílek, F., Petr, J. 2015. Endogenously produced hydrogen sulfide is involved in porcine oocyte maturation in vitro. Nitric Oxide: Biology and Chemistry. 51, 24–35. doi: 10.1016/j.niox.2015.09.007.
• Nevoral, J., Petr, J., Gélaude, A., Bodart, J.-F., Kučerová-Chrpová, V., Sedmíková, M., Krejčová, T., Kolbabová, T., Dvořáková, M., Vyskočilová, A., Weingartová, I., Křivohlávková, L., Žalmanová, T., Jílek, F. 2014. Dual effects of hydrogen sulfide donor on meiosis and cumulus expansion of porcine cumulus-oocyte complexes. PLoS One. 9(7), e99613. doi: 10.1371/journal.pone.0099613.