Call for Ph.D. Study in Reproductive Medicine

Laboratory of Reproductive Medicine calls for Ph.D. student (since October 1st, 2022) – Mitochondrial biogenesis during oocyte development and early embryonic development (supervisor: assoc. prof. Jan NEVORAL, Ph.D.).

Briefly

Mitochondria are inherited down to the maternal lineage and, therefore, oocyte mitochondrial health is essential for the fitness of offspring. We hypothesize that the transcription of mitochondrial (mt) DNA is essential for the renewal of maternal mitochondria and the elimination of mtDNA mutations. The model of conditional knock-out mouse will be used for the study of TFAM, transcriptional factor A, mitochondrial. Oocytes and early embryos will be produced and analyzed via immunostaining and mtDNA sequencing. The knowledge will explain causes of infertility associated with advanced maternal age, and improve assisted reproductive techniques.

Requirements

  • MSc. In life sciences
  • Basic laboratory experience
  • Entusiasm and good spirit

What can you expect?

  • International team
  • High-tech lab equipment
  • Traveling and fellowships

Used techniques

  • Cre-Lox crossbreeding of knock-out mice
  • In vitro a in vivo embryo production
  • Imunocytochemistry, confocal scope, and image analysis
  • mtDNA amplification and sequencing

    References

    Contact: Tato e-mailová adresa je chráněna před spamboty. Pro její zobrazení musíte mít povolen Javascript.

    Submission deadline: April 30, 2022

  • Kontakt

    Univerzita Karlova
    Lékařská fakulta v Plzni
    Biomedicínské centrum

    alej Svobody 1655/76
    323 00 Plzeň – Severní Předměstí

    T: +420 377 593 810
    E: Tato e-mailová adresa je chráněna před spamboty. Pro její zobrazení musíte mít povolen Javascript.

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